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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and <t>H5N1</t> HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.
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P20-H5 hydrogel vaccines improve humoral response to HexaPro and H5N1 HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.

Journal: Biomaterials science

Article Title: Enabling Global Access to Potent Subunit Vaccines with a Simple and Scalable Injectable Hydrogel Platform

doi: 10.1039/d5bm01131k

Figure Lengend Snippet: P20-H5 hydrogel vaccines improve humoral response to HexaPro and H5N1 HA-based subunit vaccines relative to emulsion-based formulations. a) Timeline of mouse immunizations and blood collection. Mice were immunized at week 0 and week 8, and serum was collected weekly to determine antigen-specific IgG endpoint titers. b) Anti-Spike IgG titers before and after boosting of P20-H5 and bolus HexaPro vaccines. P values are listed above each timepoint. c) AUC of anti-Spike IgG endpoint titers from week 0 to week 12. d) Anti-H5N1 HA IgG titers before and after boosting of P20-H5 and emulsion-based H5N1 HA vaccines. P values are listed above each timepoint. e) AUC of anti-H5N1 HA IgG endpoint titers from week 0 to week 12.

Article Snippet: Influenza A H5N1 (A/Indonesia/5/2005) Hemagglutinin / HA Protein (His Tag) (H5N1-HA) and SARS-CoV-2 spike protein (40589-V08H4) (spike protein) were purchased from Sino Biological.

Techniques: Vaccines, Emulsion

Germinal center responses to P20-H5 and bolus vaccines. a) Mice were immunized with vaccines at week 0 and draining lymph nodes were excised for flow cytometry analysis at week 3. b,c) Count of (b) GCBCs (CD45 + CD11b − CD19 + CD95 + CD38 − ) or (c) T FH cells (CD45 + CD11b − CD19 − CD3 + CD4 + CXCR5 + PD-1 + FOXP3 − Bcl6 + ) in dLNs three weeks post-prime immunization with WT SARS-CoV-2 vaccine (n = 7). d, e) Count of (d) GCBCs (CD45 + CD11b − CD19 + CD95 + CD38 − ) or (e) T FH cells (CD45 + CD11b − CD19 − , CD3 + CD4 + CXCR5 + PD-1 + FOXP3 − Bcl6 + ) in dLNs three weeks post-prime immunization with H5N1 influenza vaccines (n = 7)

Journal: Biomaterials science

Article Title: Enabling Global Access to Potent Subunit Vaccines with a Simple and Scalable Injectable Hydrogel Platform

doi: 10.1039/d5bm01131k

Figure Lengend Snippet: Germinal center responses to P20-H5 and bolus vaccines. a) Mice were immunized with vaccines at week 0 and draining lymph nodes were excised for flow cytometry analysis at week 3. b,c) Count of (b) GCBCs (CD45 + CD11b − CD19 + CD95 + CD38 − ) or (c) T FH cells (CD45 + CD11b − CD19 − CD3 + CD4 + CXCR5 + PD-1 + FOXP3 − Bcl6 + ) in dLNs three weeks post-prime immunization with WT SARS-CoV-2 vaccine (n = 7). d, e) Count of (d) GCBCs (CD45 + CD11b − CD19 + CD95 + CD38 − ) or (e) T FH cells (CD45 + CD11b − CD19 − , CD3 + CD4 + CXCR5 + PD-1 + FOXP3 − Bcl6 + ) in dLNs three weeks post-prime immunization with H5N1 influenza vaccines (n = 7)

Article Snippet: Influenza A H5N1 (A/Indonesia/5/2005) Hemagglutinin / HA Protein (His Tag) (H5N1-HA) and SARS-CoV-2 spike protein (40589-V08H4) (spike protein) were purchased from Sino Biological.

Techniques: Vaccines, Flow Cytometry